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KMID : 1189420070200020036
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2007 Volume.20 No. 2 p.36 ~ p.46
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Anti-inflammatory effect of various solvent extract from Atractylodes japonica on Lipopolysaccharide-induced Inflammation in BV2 cells
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Hur Inn-Hee
Sim Sung-Yong
Kim Kyung-Jun
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Abstract
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Objective: In this study, the effect of Atractylodes japonica against LPS induced inflammation in mouse microglia BV2 cells was investigated.
Method: Microglia BV2 Cells viability was determined using the MTT assay. We used water, ethanol extract from Atractylodes japonica and studied on the anti-inflammatory effect of lipopolysaccharide-induced inflammation using reverse transcription polymerase chain reaction (RT-PCR), western blot, and nitric oxide detection on mouse microglia BV2 cells.
Result: The MTT assay revealed that it’s extract has no significant cytotoxicity in the microglia BV2 cell. Various solvent extract from Atractylodes japonica inhibited nitrite production, iNOS protein and mRNA expression levels. And also it’s extracts significantly reduced lipopolysaccharide-induced COX-2 activation in RT-PCR and western blot in lipopolysaccharide-induced microglia BV2 cells
Conclusion: In this study, it’s extracts was shown to suppress NO production by inhibiting iNOS expression and COX-2 activity. With this effects of anti-inflammation, we suggests that, it’s extracts may be a useful candidate for the development of a drug on the related inflammatory diseases in brain.
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KEYWORD
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Atractylodesjaponica, nitricoxide, induciblenitricoxidesynthase, cyclooxygenase-2
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